Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry

Seon Hwa Lee, Tomoyuki Oe, Ian A. Blair

Research output: Contribution to conferencePaper

Abstract

The development of an assay for the analysis of etheno 2′- Deoxygyanosine (dGuo) adducts from biological matrices was described. The use of isotope dilution liquid chromatography/atomospheric pressure chemical ionization/mass spectrometry (LC/APCI/MA) was also discussed. The reaction between 13-hydroperoxyoctadecadienoic acid (13-HPODE) and dGuo in the presence of vitamin C resulted in the fomation of three products; etheno-dGuo, carboxynonanone etheno-dGuo, and heptanone ehteno-dGuo. The results show that the mass spectrum of the most polar adduct (10.4 min) revealed an intense protonated molecular ion (H +) along with a BH 2 + ion at m/z 176.

Original languageEnglish
Pages635-636
Number of pages2
Publication statusPublished - 2002 Dec 1
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/202/6/6

ASJC Scopus subject areas

  • Spectroscopy

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  • Cite this

    Lee, S. H., Oe, T., & Blair, I. A. (2002). Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry. 635-636. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.