Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The Drosophila melanogaster wing imaginal disc is an epithelial sac that exhibits dramatic tissue growth during the larval stage. With its simple morphology and accessibility of genetic tools, studies using the wing disc have contributed to the understanding of the mechanisms of epithelial homeostasis including the control of mitotic spindle orientation. This chapter describes a detailed protocol for analyzing epithelial architecture and planar orientation of the mitotic spindle in the wing disc epithelium. The rapid dissection method, effective immunostaining, and mounting tips described here facilitate genetic and cell biological studies of the wing disc and can be applied to a wide array of studies using various Drosophila tissues.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages51-62
Number of pages12
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2346
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell division
  • Confocal microscopy
  • Drosophila
  • Epithelial architecture
  • Immunostaining
  • Planar spindle orientation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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