Analysis of ciliary motion and the axonemal structure in the mouse respiratory cilia

Hironori Ueno, Takuji Ishikawa, Khanh Huy Bui, Kohsuke Gonda, Takashi Ishikawa, Takami Yamaguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mucociliary clearance on the surface of the tracheal lumen is an important component of lung defense against dust mites and viruses. However, the axonemal structure that achieves effective ciliary motion and the mechanisms by which discretely distributed ciliary cells generate directional flow are unknown. In this study, we examined individual ciliary motion with 7-9-nm spatial precision by labeling the ciliary tip with quantum dots, and detected an asymmetric beating pattern. Cryo-electron tomography revealed that the densities of two inner dynein arms were missing from at least two doublet microtubules in the axonemal structure. Although the flow directions generated by individual ciliated cells were unsteady and diverse, the time- and space-averaged velocity field was found to be directional. These results indicate that the asymmetric ciliary motion is driven by the asymmetric axonemal structure, and it generates overall directional flow from the lungs to the oropharynx on sparsely distributed ciliated cells.

Original languageEnglish
Title of host publicationASME 2012 Summer Bioengineering Conference, SBC 2012
Pages1259-1260
Number of pages2
DOIs
Publication statusPublished - 2012 Dec 1
EventASME 2012 Summer Bioengineering Conference, SBC 2012 - Fajardo, Puerto Rico
Duration: 2012 Jun 202012 Jun 23

Publication series

NameASME 2012 Summer Bioengineering Conference, SBC 2012

Other

OtherASME 2012 Summer Bioengineering Conference, SBC 2012
CountryPuerto Rico
CityFajardo
Period12/6/2012/6/23

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Ueno, H., Ishikawa, T., Bui, K. H., Gonda, K., Ishikawa, T., & Yamaguchi, T. (2012). Analysis of ciliary motion and the axonemal structure in the mouse respiratory cilia. In ASME 2012 Summer Bioengineering Conference, SBC 2012 (pp. 1259-1260). (ASME 2012 Summer Bioengineering Conference, SBC 2012). https://doi.org/10.1115/SBC2012-80232