Analysis of cellular behavior of attachment and spreading by QCM combined with micro channel

Etsuko Watarai, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara

Research output: Contribution to conferencePaper

Abstract

In the recent tissue engineering, the necessity of the control of cellular behavior is increasing more and more. In this study, we quantified cell attachment and spreading by unit of cell by using artificial cell membrane, micro channel and QCM, and observed cellular behavior, for aiming at the clarification of the way of the control of cellular behavior and mechanism of a cell. Micro channel is made of polydimethylsiloxane (PDMS) and surface in channel is coated with 2-methacryloyloxyehyl phosphorylcholine(MPC) polymer, which was bioinspired from the cell membrane, to inhibit the non-specific cell adhesion on the surface. By using this micro channel, we succeeded in taking out a single cell. From measurement result by QCM of 50000,5000,500cells/ml L929 cell suspension, cells grew steadily on gold electrode, and the resistance change of QCM electrode are related to the number of adherent cells. From the results, it is concluded that the single cell analysis system combined by QCM and micro-channel which have the function of cell separation is constructed.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Artificial cell membrane
  • Micro channel
  • QCM(Quartz Crystal Microbalance)

ASJC Scopus subject areas

  • Engineering(all)

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    Watarai, E., Konno, T., Takai, M., & Ishihara, K. (2006). Analysis of cellular behavior of attachment and spreading by QCM combined with micro channel. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.