Analysis of Ca2+currents in spermatocytes from mice lacking Cav2.3 (α1E) Ca2+channel

Yu Sakata, Hironao Saegusa, Shuqin Zong, Makoto Osanai, Takayuki Murakoshi, Yasufumi Shimizu, Tetsuo Noda, Takeshi Aso, Tsutomu Tanabe

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

In mammalian male germ-line cells, low-voltage-activated (LVA) Ca2+current has been identified and its electrophysiological properties have been studied. To investigate whether α12.3 (α1E) subunit of the voltage-dependent Ca2+channel codes for the LVA current, whole-cell patch clamp and following reverse transcription-polymerase chain reaction (RT-PCR) experiments were performed in pachytene spermatocytes from Cav2.3+/+ and Cav2.3-/- mice. Whole-cell current in acutely dissociated pachytene spermatocytes from Cav2.3+/+ and Cav2.3-/- mice displayed a typical profile of LVA Ca2+currents and kinetics with no significant differences. Single-cell RT-PCR revealed the expression of Cacna1g in the pachytene spermatocytes from Cav2.3+/+ and Cav2.3-/- mice in which LVA Ca2+currents were actually recorded. These results suggest that the Cav2.3 channel makes no detectable contribution to the LVA Ca2+current in the pachytene spermatocyte. Instead, Cav3 family such as Cav3.1 may be the likely candidates responsible for the LVA currents in pachytene spermatocytes.

Original languageEnglish
Pages (from-to)1032-1036
Number of pages5
JournalBiochemical and biophysical research communications
Volume288
Issue number4
DOIs
Publication statusPublished - 2001 Nov 9

Keywords

  • Ca2.3
  • Ca3.1
  • Knockout mouse
  • Low-voltage-activated Cacurrents
  • Pachytene spermatocyte
  • R-type
  • T-type
  • Voltage-dependent Cachannel
  • α
  • α

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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