Analysis of c-myc oncogene in human esophageal carcinoma: Immunohistochemistry, in situ hybridization and Northern and Southern blot studies

S. Miyazaki, H. Sasno, K. Shiga, T. Sawai, T. Nishihira, H. Okamoto, S. Mori

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We have examined the c-myc gene expression and the gene organization in resected human esophageal squamous cell carcinomas, and in the adjacent normal esophageal mucosa from 20 patients udergoing radical surgery. Immunohistochemistry of p62(c-myc) was compared with that of proliferating cell nuclear antigen (PCNA) in order to examine the biologic significance of p62(c-myc). Relative c-myc expression detected by Northern blot analysis ranged from 0.41 to 2.8, but the degree of c-myc expression did not correlate with other clinicopathological prognostic parameters. In sity hybridization localized the elevated c-myc mRNA expression to tumor cells and basal and parabasal cells of the adjacent normal mucosa. Immunohistochemistry showed altered localization of p62(c-myc), i.e., both cytoplasmic and nuclear immunostaining in advanced carcinomas. c-myc immunoreactivity exhibited wider distribution compared with that of PCNA, a cell cycle related antigen, which may indicate induction of cell proliferation by p62(c-myc). DNA hybridization showed mild amplification in one out of 17 tumors and no evidence of gene rearrangement. There was no distinct correlation between the results of Northern or Southern blot analysis and the results of in situ hybridization or immunohistochemistry. Gene alteration of the c-myc locus, as well as overexpression of the c-myc oncogene, appeared to be limited, and analysis of the c-myc gene yielded limited prognostic value in human esophageal carcinomas.

Original languageEnglish
Pages (from-to)1747-1755
Number of pages9
JournalAnticancer research
Volume12
Issue number5
Publication statusPublished - 1992

Keywords

  • Carcinoma
  • Esophagus
  • Immunohistochemistry
  • In situ hybridization
  • Northern blot
  • Southern blot
  • c-myc

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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