Analysis of appositional bone formation using a novel rat experimental model

D. Nakajima, S. Kamakura, M. Nakamura, O. Suzuki, S. Echigo, Y. Sasano

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Aim: To analyze the process of appositional bone formation using our original rat experimental model. Materials and methods: Rats were anesthetized and a ring made of polytetrafluorethylene was placed on the parietal bone surface in the surgical procedure. The time course of appositional bone formation was analyzed with histomorphometry and in situ hybridization for type I collagen and bone sialoprotein. Results: The rat experimental model allowed new bone to be formed on the pre-existing bone surface and persist for 12 weeks. We demonstrated that bone is apposed actively for the first 4 weeks and less actively thereafter. Conclusions: The experimental model may contribute to biological analysis for appositional bone formation expected to occur in clinical procedures such as alveolar bone augmentation and sinus lifting.

Original languageEnglish
Pages (from-to)308-313
Number of pages6
JournalOral Diseases
Issue number4
Publication statusPublished - 2008 May 1


  • Appositional bone formation
  • Bone sialoprotein
  • Histomorphometry
  • In situ hybridization
  • Rat experimental model
  • Type I collagen

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)


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