Abstract
Antibodies against human T-cell lymphotropic virus type I (HTLV-I) in the sera from 60 patients with adult T-cell leukemia and 21 carriers who were suspected of having HTLV-I infection were investigated by indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), membrane immunofluorescence assay (MIA) and strip radio immunoassay based on the western blotting technique (SRIA). The sera of 2 of the carriers who were seropositive in IFA and ELISA were negative in MIA and did not react with virus-specific proteins by SRIA. Two sera were negative in IFA and ELISA. These sera were positive in MIA and reacted with only the envelope-related glycoprotein (gp46) and not with gag-related proteins (p28, p24, p19) by SRIA. These findings suggest that the main antigens defined by IFA and ELISA are gag-related proteins and some sera which do not contain anti-HTLV-I antibodies give false-positive results because of the reaction to unknown cellular components. Also some sera may have antibodies against only envelope glycoproteins, and these sera may give false-negative results in IFA and ELISA.
| Original language | English |
|---|---|
| Pages (from-to) | 605-610 |
| Number of pages | 6 |
| Journal | Leukemia Research |
| Volume | 10 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1986 |
| Externally published | Yes |
Keywords
- ATL
- ELISA
- HTLV-I
- envelope glycoprotein
- gag protein
- strip radioimmunoassay
- western blotting
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research
