Analysis for localization of steroid sulfatase in human tissues

Takashi Suzuki, Yasuhiro Miki, Tsuyoshi Fukuda, Taisuke Nakata, Takuya Moriya, Hironobu Sasano

Research output: Contribution to journalReview articlepeer-review

9 Citations (Scopus)

Abstract

Human steroid sulfatase (STS) is an enzyme that hydrolyzes several sulfated steroids, such as estrone sulfate, dehydroepiandrosterone sulfate, and cholesterol sulfate, and results in the production of active substances. STS has been demonstrated in human breast cancer tissues and is considered to be involved in intratumoral estrogen production. It is very important to analyze the cellular distribution of STS with accuracy in human tissues in order to obtain a better understanding of the biological significance of STS. Therefore, this chapter describes several morphological approaches used to study the localization of STS, including immunohistochemistry, mRNA in situ hybridization, and laser capture microdissection/reverse transcription-polymerase chain reaction, in human tissues.

Original languageEnglish
Article number18
Pages (from-to)303-316
Number of pages14
JournalMethods in enzymology
Volume400
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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