Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.

Atsushi Kobayashi; Motomitsu Kitaoka; Kiyoshi Hayashi

Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.

Atsushi Kobayashi, Motomitsu Kitaoka, Kiyoshi Hayashi

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We determined nucleotide incorporation specificity of four thermostable DNA polymerases opposite deoxyinosine in order to evaluate the mutagenic effect of the presence of a consecutive deoxyinosine sequence during PCR amplification. Deoxycytosine was the only incorporated nucleotide opposite deoxyinosine during PCR amplification, irrespective of origin of DNA polymerase.

Original language	English
Pages (from-to)	225-226
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	48
Publication status	Published - 2004

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.",

abstract = "We determined nucleotide incorporation specificity of four thermostable DNA polymerases opposite deoxyinosine in order to evaluate the mutagenic effect of the presence of a consecutive deoxyinosine sequence during PCR amplification. Deoxycytosine was the only incorporated nucleotide opposite deoxyinosine during PCR amplification, irrespective of origin of DNA polymerase.",

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T1 - Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.

AU - Kobayashi, Atsushi

AU - Kitaoka, Motomitsu

AU - Hayashi, Kiyoshi

PY - 2004

Y1 - 2004

N2 - We determined nucleotide incorporation specificity of four thermostable DNA polymerases opposite deoxyinosine in order to evaluate the mutagenic effect of the presence of a consecutive deoxyinosine sequence during PCR amplification. Deoxycytosine was the only incorporated nucleotide opposite deoxyinosine during PCR amplification, irrespective of origin of DNA polymerase.

AB - We determined nucleotide incorporation specificity of four thermostable DNA polymerases opposite deoxyinosine in order to evaluate the mutagenic effect of the presence of a consecutive deoxyinosine sequence during PCR amplification. Deoxycytosine was the only incorporated nucleotide opposite deoxyinosine during PCR amplification, irrespective of origin of DNA polymerase.

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JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 48

ER -

Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.

Abstract

ASJC Scopus subject areas

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