Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.

Atsushi Kobayashi, Motomitsu Kitaoka, Kiyoshi Hayashi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We determined nucleotide incorporation specificity of four thermostable DNA polymerases opposite deoxyinosine in order to evaluate the mutagenic effect of the presence of a consecutive deoxyinosine sequence during PCR amplification. Deoxycytosine was the only incorporated nucleotide opposite deoxyinosine during PCR amplification, irrespective of origin of DNA polymerase.

Original languageEnglish
Pages (from-to)225-226
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number48
Publication statusPublished - 2004

ASJC Scopus subject areas

  • Medicine(all)

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