TY - JOUR
T1 - Analyses of Dansyl and Dabsyl Amino Acids by Reverse-Phase High-Performance Liquid Chromatography
AU - Muramoto, Koji
AU - Kamiya, Hisao
PY - 1985/1/1
Y1 - 1985/1/1
N2 - Dansyl and dabsyl amino acids were separated by reverse-phase high-performance liquid chromatography on a short column (4.6�-50mm) packed with 3��m ODS particles using a gradient formed from acetone and 10 mM sodium phosphate buffer, pH 6.5 or 7. 0. The light absorption of the derivatives was used for the detection giving a sensitivity of less than 50 pmol for a dansyl derivative or 10 pmol for a dabsyl derivative. This system was applicable to the amino acid analyses, amino-terminal analyses, and carboxyl-terminal analyses with less than 1 nmol of peptides.
AB - Dansyl and dabsyl amino acids were separated by reverse-phase high-performance liquid chromatography on a short column (4.6�-50mm) packed with 3��m ODS particles using a gradient formed from acetone and 10 mM sodium phosphate buffer, pH 6.5 or 7. 0. The light absorption of the derivatives was used for the detection giving a sensitivity of less than 50 pmol for a dansyl derivative or 10 pmol for a dabsyl derivative. This system was applicable to the amino acid analyses, amino-terminal analyses, and carboxyl-terminal analyses with less than 1 nmol of peptides.
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U2 - 10.2331/suisan.51.817
DO - 10.2331/suisan.51.817
M3 - Article
AN - SCOPUS:85004672113
VL - 51
SP - 817
EP - 824
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 5
ER -