Analyses of Dansyl and Dabsyl Amino Acids by Reverse-Phase High-Performance Liquid Chromatography

Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Dansyl and dabsyl amino acids were separated by reverse-phase high-performance liquid chromatography on a short column (4.6�-50mm) packed with 3��m ODS particles using a gradient formed from acetone and 10 mM sodium phosphate buffer, pH 6.5 or 7. 0. The light absorption of the derivatives was used for the detection giving a sensitivity of less than 50 pmol for a dansyl derivative or 10 pmol for a dabsyl derivative. This system was applicable to the amino acid analyses, amino-terminal analyses, and carboxyl-terminal analyses with less than 1 nmol of peptides.

    Original languageEnglish
    Pages (from-to)817-824
    Number of pages8
    JournalNIPPON SUISAN GAKKAISHI
    Volume51
    Issue number5
    DOIs
    Publication statusPublished - 1985 Jan 1

    ASJC Scopus subject areas

    • Aquatic Science

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