Anaerobic treatment of N, N-dimethylformamide-containing wastewater by co-culturing two sources of inoculum

Zhe Kong, Lu Li, Rei Kurihara, Kengo Kubota, Yu You Li

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

The complete methanogenic degradation of N, N-dimethylformamide (DMF) was achieved in this study. Initially, DMF was found to be feebly degradable by a lab-scale submerged anaerobic membrane bioreactor (SAnMBR) using normal anaerobic digestion sludge (ADS) even after 120-day's culturing. However, aerobic DMF-degrading activated sludge (AS) was rapidly cultivated in a continuous aeration reactor (CAR). A specially designed anaerobic co-cultured sludge (ACS) made by artificially mixing AS with ADS was successfully domesticated by a long term repeated batch experiment. The results demonstrated that ACS could effectively degrade over 5000 mg L−1 DMF for methane recovery. The metabolic pathway and stoichiometric equation of DMF methanogenic degradation were also revealed and verified in detail. It was confirmed that under the anaerobic condition, with the help of enzyme, DMF converts into dimethylamine and formic acid, and the intermediates are effectively fermented through methylotrophic/hydrogenotrophic methanogenesis. Analysis of the microbial community suggested that some facultatively anaerobic bacteria played the key roles in methanogenic degradation due to their DMF-hydrolyzing ability. By co-culturing two sources of inoculum under the anaerobic condition, the symbiosis of facultatively anaerobic DMF-hydrolyzing bacteria and methylotrophic/hydrogenotrophic methanogens makes methanogenic degradation of DMF available. This study also provides a novel sludge cultivation method for anaerobic treatment of degradation-resistant organics.

Original languageEnglish
Pages (from-to)228-239
Number of pages12
JournalWater Research
Volume139
DOIs
Publication statusPublished - 2018 Aug 1

Keywords

  • Co-culture
  • Enzyme
  • Facultative anaerobes
  • Hydrolysis
  • Methanogenic degradation
  • N, N-dimethylformamide

ASJC Scopus subject areas

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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