Abstract
An unmediated hydrogen peroxide sensor is designed in this paper by employing a hemoglobin-SDS film modified electrode. Hemoglobin exhibits direct (unmediated) electrochemistry at the modified electrode. The protein also shows elegant catalytic activity towards the electrochemical reduction of hydrogen peroxide. Consequently, a prototype hydrogen peroxide sensor is prepared. Under optimum conditions, this sensor provides a linear response over the hydrogen peroxide concentrations in the range of 1x10-5 ~ 1 x 10-4 mol/L. The detection limit was 2 x 10-6 mol/L The relative standard deviation was 4.2% for 6 successive determinations of the hydrogen peroxide at 1xl0-5 mol/L. This configuration is shown to be sensitive, stable and easily fabricated. It might be useful in the biological and industrial fields.
Original language | English |
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Pages (from-to) | 2631-2644 |
Number of pages | 14 |
Journal | Analytical Letters |
Volume | 33 |
Issue number | 13 |
DOIs | |
Publication status | Published - 2000 Jan 1 |
Keywords
- Hemoglobin
- Hydrogen peroxide sensor
- SDS
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Spectroscopy
- Clinical Biochemistry
- Biochemistry, medical
- Electrochemistry