An ultramicroelectrode for determination of intracellular oxygen. Light-irradiation-induced change in oxygen concentration in an algal protoplast

Tomokazu Matsue, Shinji Koike, Takayuki Abe, Takeyuki Itabashi, Isamu Uchida

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

An oxygen ultramicroelectrode was fabricated by pyrolysis of butane inside a glass capillary, followed by electrodeposition of Pt. The intracellular determination of oxygen in an algal protoplast (diameter, approx. 80 μm) with the ultramicroelectrode indicated that at a light intensity of 25 klx, the oxygen efflux from a single protoplast caused by the light reactions is approx. 5·10-13 mol/s and that corresponding to the dark reactions approx, 1 · 10-12 mol/s.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1101
Issue number1
DOIs
Publication statusPublished - 1992 Jul 6

Keywords

  • Alga
  • Intracellular oxygen
  • Light irradiation
  • Oxygen
  • Protoplast
  • Ultramicroelectrode

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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