An RNA biding protein, Y14 interacts with and modulates STAT3 activation

Norihiko Ohbayashi, Naohisa Taira, Shiho Kawakami, Sumihito Togi, Noriko Sato, Osamu Ikeda, Shinya Kamitani, Ryuta Muromoto, Yuichi Sekine, Tadashi Matsuda

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine-phosphorylation, dimerization, and nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT3 activation, we performed yeast two-hybrid screening. We identified Y14, an RNA-binding protein, as a novel STAT3 binding partner. Y14 bound to STAT3 through the C-terminal region of STAT3 in vivo. Importantly, small-interfering RNA-mediated reduction of endogenous Y14 expression decreased IL-6-induced tyrosine-phosphorylation, nuclear accumulation, and DNA-binding activity of STAT3, as well as IL-6/STAT3-dependent gene expression. These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3.

    Original languageEnglish
    Pages (from-to)475-479
    Number of pages5
    JournalBiochemical and biophysical research communications
    Volume372
    Issue number3
    DOIs
    Publication statusPublished - 2008 Aug 1

    Keywords

    • Cytokine
    • Gene expression
    • RNA-binding protein
    • STAT3
    • Signal transduction
    • Y14

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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  • Cite this

    Ohbayashi, N., Taira, N., Kawakami, S., Togi, S., Sato, N., Ikeda, O., Kamitani, S., Muromoto, R., Sekine, Y., & Matsuda, T. (2008). An RNA biding protein, Y14 interacts with and modulates STAT3 activation. Biochemical and biophysical research communications, 372(3), 475-479. https://doi.org/10.1016/j.bbrc.2008.05.073