An oxygen responsive microparticle-patterned hydrogel sheet for enzyme activity imaging

Kuniaki Nagamine, Shuntaro Ito, Mai Takeda, Shingo Otani, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible Polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.

Original languageEnglish
Pages (from-to)318-320
Number of pages3
JournalElectrochemistry
Volume80
Issue number5
DOIs
Publication statusPublished - 2012 May

Keywords

  • Electrohydrodynamics
  • Microfluidic device
  • Oxygen imaging sheet

ASJC Scopus subject areas

  • Electrochemistry

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