An open-source add-on evom® device for real-time transepithelial/endothelial electrical resistance measurements in multiple transwell samples

Bibek Raut, Li Jiun Chen, Takeshi Hori, Hirokazu Kaji

Research output: Contribution to journalArticlepeer-review

Abstract

This study provides design of a low-cost and open source add-on device that enhances the functionality of the popular EVOM® instrument for transepithelial/endothelial electrical resistance (TEER) measurement. The original EVOM® instrument is designed for measuring TEER in transwell samples manually using a pair of Ag/AgCl electrodes. The inconsistency in electrode placement, temperature variation, and a typically large (12–24 h) time interval between measurements result in large data variabilities. Thus, to solve the current limitation of the EVOM® instrument, we built an add-on device using a custom designed electronic board and a 3D printed electrode holder that allowed automated TEER measurements in multiple transwell samples. To demonstrate the functionality of the device prototype, we monitored TEER in 4 transwell samples containing retinal cells (ARPE-19) for 67 h. Furthermore, by monitoring temperature of the cell culture medium, we were able to detect fluctuations in TEER due to temperature change after the medium change process, and were able to correct the data offset. Although we demonstrated the use of our add-on device on EVOM® instrument only, the concept (multiplexing using digitally controlled relays) and hardware (custom data logger) presented here can be applied to more advanced TEER instruments to improve the performance of those devices.

Original languageEnglish
Article number282
Pages (from-to)1-13
Number of pages13
JournalMicromachines
Volume12
Issue number3
DOIs
Publication statusPublished - 2021 Mar

Keywords

  • 4-electrode resistance
  • Chopstick electrode
  • EVOM
  • Multi-array switching
  • Ohmic resistance
  • Real-time TEER measurement
  • Tight junction

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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