TY - JOUR
T1 - An NK and T cell enhancer lies 280 kilobase pairs 3' to the Gata3 structural gene
AU - Hosoya-Ohmura, Sakie
AU - Lin, Yu Hsuan
AU - Herrmann, Mary
AU - Kuroha, Takashi
AU - Rao, Arvind
AU - Moriguchi, Takashi
AU - Lim, Kim Chew
AU - Hosoya, Tomonori
AU - Engel, James Douglas
PY - 2011/5
Y1 - 2011/5
N2 - Transcription factor GATA-3 is vital for multiple stages of T cell and natural killer (NK) cell development, and yet the factors that directly regulate Gata3 transcription during hematopoiesis are only marginally defined. Here, we show that neither of the Gata3 promoters, previously implicated in its tissue-specific regulation, is alone capable of directing Gata3 transcription in T lymphocytes. In contrast, by surveying large swaths of DNA surrounding the Gata3 locus, we located a cis element that can recapitulate aspects of the Gata3-dependent T cell regulatory program in vivo. This element, located 280 kbp 3' to the structural gene, directs both T cell- and NK cell-specific transcription in vivo but harbors no other tissue activity. This novel, distant element regulates multiple major developmental stages that require GATA-3 activity.
AB - Transcription factor GATA-3 is vital for multiple stages of T cell and natural killer (NK) cell development, and yet the factors that directly regulate Gata3 transcription during hematopoiesis are only marginally defined. Here, we show that neither of the Gata3 promoters, previously implicated in its tissue-specific regulation, is alone capable of directing Gata3 transcription in T lymphocytes. In contrast, by surveying large swaths of DNA surrounding the Gata3 locus, we located a cis element that can recapitulate aspects of the Gata3-dependent T cell regulatory program in vivo. This element, located 280 kbp 3' to the structural gene, directs both T cell- and NK cell-specific transcription in vivo but harbors no other tissue activity. This novel, distant element regulates multiple major developmental stages that require GATA-3 activity.
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U2 - 10.1128/MCB.05065-11
DO - 10.1128/MCB.05065-11
M3 - Article
C2 - 21383068
AN - SCOPUS:79955364415
VL - 31
SP - 1894
EP - 1904
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 9
ER -