An NK and T cell enhancer lies 280 kilobase pairs 3' to the Gata3 structural gene

Sakie Hosoya-Ohmura, Yu Hsuan Lin, Mary Herrmann, Takashi Kuroha, Arvind Rao, Takashi Moriguchi, Kim Chew Lim, Tomonori Hosoya, James Douglas Engel

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Transcription factor GATA-3 is vital for multiple stages of T cell and natural killer (NK) cell development, and yet the factors that directly regulate Gata3 transcription during hematopoiesis are only marginally defined. Here, we show that neither of the Gata3 promoters, previously implicated in its tissue-specific regulation, is alone capable of directing Gata3 transcription in T lymphocytes. In contrast, by surveying large swaths of DNA surrounding the Gata3 locus, we located a cis element that can recapitulate aspects of the Gata3-dependent T cell regulatory program in vivo. This element, located 280 kbp 3' to the structural gene, directs both T cell- and NK cell-specific transcription in vivo but harbors no other tissue activity. This novel, distant element regulates multiple major developmental stages that require GATA-3 activity.

Original languageEnglish
Pages (from-to)1894-1904
Number of pages11
JournalMolecular and cellular biology
Volume31
Issue number9
DOIs
Publication statusPublished - 2011 May

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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