An interface chip connection between capillary electrophoresis and thermal lens microscope

Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori

Research output: Contribution to journalReview articlepeer-review

36 Citations (Scopus)

Abstract

A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 × 10-7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 × 10-8 M, which was 100 times lower than that of conventional absorbance detection.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalElectrophoresis
Volume24
Issue number1-2
DOIs
Publication statusPublished - 2003 Jan
Externally publishedYes

Keywords

  • Capillary electrophoresis
  • Dead volume-free connection
  • Microchip
  • Microfabrication
  • Thermal lens microscope

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'An interface chip connection between capillary electrophoresis and thermal lens microscope'. Together they form a unique fingerprint.

Cite this