TY - JOUR
T1 - An increase in unsaturation of fatty acids in phosphatidylglycerol from leaves improves the rates of photosynthesis and growth at low temperatures in transgenic rice seedlings
AU - Ariizumi, Tohru
AU - Kishitani, Sachie
AU - Inatsugi, Rie
AU - Nishida, Ikuo
AU - Murata, Norio
AU - Toriyama, Kinya
N1 - Funding Information:
This work was supported by a grant for Recombinant Plant Project from the Ministry of Agriculture, Forestry and Fisheries, Japan.
PY - 2002
Y1 - 2002
N2 - The level of cis-unsaturated fatty acids in phosphatidylglycerol (PG) from rice leaves was genetically altered from 19.3% in the wild-type to 29.4 and 32.0% in T1 plants segregated with cDNAs for glycerol-3-phosphate acyltransferase of chloroplasts (GPAT; EC 2.3.1.15) from Arabidopsis (+AGPAT plant) and spinach (+SGPAT plant), respectively; and to 21.4% in a non-transformant segregated from +SGPAT plants (-SGPAT plant). In all these plants, O2 evolution from leaves was similar at 25°C and was impaired to a similar extent at 5 and 11°C. However, in parallel with the levels of cis-unsaturated fatty acids in PG, +AGPAT and +SGPAT plants showed less impaired rates of O2 evolution from leaves than the wild-type and -SGPAT plants at 14 and 17°C. In agreement with this, the fresh weight of 14-day-old seedlings increased to 571±18, 591±23, 687±32 and 705±31 mg in the wild-type, -SGPAT, +AGPAT and +SGPAT plants, respectively, after 6 weeks at 17/14°C (day/night). These results demonstrate the practical importance of the present technology with GPAT in improvement of the chilling sensitivity of crops.
AB - The level of cis-unsaturated fatty acids in phosphatidylglycerol (PG) from rice leaves was genetically altered from 19.3% in the wild-type to 29.4 and 32.0% in T1 plants segregated with cDNAs for glycerol-3-phosphate acyltransferase of chloroplasts (GPAT; EC 2.3.1.15) from Arabidopsis (+AGPAT plant) and spinach (+SGPAT plant), respectively; and to 21.4% in a non-transformant segregated from +SGPAT plants (-SGPAT plant). In all these plants, O2 evolution from leaves was similar at 25°C and was impaired to a similar extent at 5 and 11°C. However, in parallel with the levels of cis-unsaturated fatty acids in PG, +AGPAT and +SGPAT plants showed less impaired rates of O2 evolution from leaves than the wild-type and -SGPAT plants at 14 and 17°C. In agreement with this, the fresh weight of 14-day-old seedlings increased to 571±18, 591±23, 687±32 and 705±31 mg in the wild-type, -SGPAT, +AGPAT and +SGPAT plants, respectively, after 6 weeks at 17/14°C (day/night). These results demonstrate the practical importance of the present technology with GPAT in improvement of the chilling sensitivity of crops.
KW - Chilling tolerance
KW - Fatty acid composition
KW - Glycerol-3-phosphate acyltransferase
KW - Oryza sativa L.
KW - Phosphatidylglycerol
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U2 - 10.1093/pcp/pcf087
DO - 10.1093/pcp/pcf087
M3 - Article
C2 - 12154137
AN - SCOPUS:0036343017
VL - 43
SP - 751
EP - 758
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -