An increase in unsaturation of fatty acids in phosphatidylglycerol from leaves improves the rates of photosynthesis and growth at low temperatures in transgenic rice seedlings

Tohru Ariizumi, Sachie Kishitani, Rie Inatsugi, Ikuo Nishida, Norio Murata, Kinya Toriyama

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

The level of cis-unsaturated fatty acids in phosphatidylglycerol (PG) from rice leaves was genetically altered from 19.3% in the wild-type to 29.4 and 32.0% in T1 plants segregated with cDNAs for glycerol-3-phosphate acyltransferase of chloroplasts (GPAT; EC 2.3.1.15) from Arabidopsis (+AGPAT plant) and spinach (+SGPAT plant), respectively; and to 21.4% in a non-transformant segregated from +SGPAT plants (-SGPAT plant). In all these plants, O2 evolution from leaves was similar at 25°C and was impaired to a similar extent at 5 and 11°C. However, in parallel with the levels of cis-unsaturated fatty acids in PG, +AGPAT and +SGPAT plants showed less impaired rates of O2 evolution from leaves than the wild-type and -SGPAT plants at 14 and 17°C. In agreement with this, the fresh weight of 14-day-old seedlings increased to 571±18, 591±23, 687±32 and 705±31 mg in the wild-type, -SGPAT, +AGPAT and +SGPAT plants, respectively, after 6 weeks at 17/14°C (day/night). These results demonstrate the practical importance of the present technology with GPAT in improvement of the chilling sensitivity of crops.

Original languageEnglish
Pages (from-to)751-758
Number of pages8
JournalPlant and Cell Physiology
Volume43
Issue number7
DOIs
Publication statusPublished - 2002

Keywords

  • Chilling tolerance
  • Fatty acid composition
  • Glycerol-3-phosphate acyltransferase
  • Oryza sativa L.
  • Phosphatidylglycerol

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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