An improved method for recovering rabies virus from cloned cDNA

Ken Ichi Inoue, Youko Shoji, Ichiro Kurane, Toshio Iijima, Takeo Sakai, Kinjiro Morimoto

    Research output: Contribution to journalArticlepeer-review

    107 Citations (Scopus)

    Abstract

    A new system for recovery of rabies virus from cDNA plasmid, the transcription of which was driven by cellular RNA polymerase II, was developed. The plasmid contains full-length viral cDNA flanked by hammerhead ribozyme and hepatitis delta ribozyme sequences, arranged downstream of the cytomegalovirus (CMV) promotor. Transfection with the full-length cDNA plasmid together with helper plasmids encoding viral N, P, and L proteins without supply of T7 RNA polymerase produced a recombinant rabies virus in several cell lines. The efficiency of recovery between the conventional T7 promotor system and the new CMV promotor system was compared using these plasmid constructs. The newly established system is applicable to various cell lines and allows rapid and efficient generation of recombinant rabies virus.

    Original languageEnglish
    Pages (from-to)229-236
    Number of pages8
    JournalJournal of Virological Methods
    Volume107
    Issue number2
    DOIs
    Publication statusPublished - 2003 Feb

    Keywords

    • Cytomegalovirus promotor
    • HEP-Flury strain
    • Rabies virus
    • Reverse genetics

    ASJC Scopus subject areas

    • Virology

    Fingerprint Dive into the research topics of 'An improved method for recovering rabies virus from cloned cDNA'. Together they form a unique fingerprint.

    Cite this