An Improved Method for Perforated Patch Recordings Using Nystatin-Fluorescein Mixture

Hiromu Yawo, Nao Chuhma

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 MΩ was readily achieved.

    Original languageEnglish
    Pages (from-to)267-273
    Number of pages7
    JournalJapanese Journal of Physiology
    Volume43
    Issue number2
    DOIs
    Publication statusPublished - 1993

    Keywords

    • nystatin
    • patch clamp

    ASJC Scopus subject areas

    • Physiology

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