An Improved Method for Perforated Patch Recordings Using Nystatin-Fluorescein Mixture

Hiromu Yawo; Nao Chuhma

doi:10.2170/jjphysiol.43.267

An Improved Method for Perforated Patch Recordings Using Nystatin-Fluorescein Mixture

Hiromu Yawo, Nao Chuhma

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 MΩ was readily achieved.

Original language	English
Pages (from-to)	267-273
Number of pages	7
Journal	Japanese Journal of Physiology
Volume	43
Issue number	2
DOIs	https://doi.org/10.2170/jjphysiol.43.267
Publication status	Published - 1993

Keywords

nystatin
patch clamp

ASJC Scopus subject areas

Physiology

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AB - This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 MΩ was readily achieved.

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KW - patch clamp

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An Improved Method for Perforated Patch Recordings Using Nystatin-Fluorescein Mixture

Abstract

Keywords

ASJC Scopus subject areas

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