An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: Its origin and application

Takashi Moriya, Ayano Kawamata, Yusuke Takahashi, Yoshiharu Iwabuchi, Naoki Kanoh

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We have developed a fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran. The reaction of NAD(P)+ with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)+. This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.

Original languageEnglish
Pages (from-to)11500-11502
Number of pages3
JournalChemical Communications
Volume49
Issue number98
DOIs
Publication statusPublished - 2013 Nov 14

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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