An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro

Noritoshi Takahashi, Haruki Kitazawa, Takeshi Shimosato, Noriyuki Iwabuchi, Jin Zhong Xiao, Keiji Iwatsuki, Sadayuki Kokubo, Tadao Saito

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5′- GCGTCGGTTTCGGTGCTCAC-3′) was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-γ (IFN-γ) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.

Original languageEnglish
Pages (from-to)461-469
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume46
Issue number3
DOIs
Publication statusPublished - 2006 Apr

Keywords

  • Bifidobacterium longum BB536
  • CpG
  • Cytokine
  • Immunoglobulin E
  • Immunostimulatory sequence oligodeoxynucleotides
  • Probiotics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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