An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro

Noritoshi Takahashi; Haruki Kitazawa; Takeshi Shimosato; Noriyuki Iwabuchi; Jin Zhong Xiao; Keiji Iwatsuki; Sadayuki Kokubo; Tadao Saito

doi:10.1111/j.1574-695X.2006.00064.x

An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro

Noritoshi Takahashi, Haruki Kitazawa, Takeshi Shimosato, Noriyuki Iwabuchi, Jin Zhong Xiao, Keiji Iwatsuki, Sadayuki Kokubo, Tadao Saito

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5′- GCGTCGGTTTCGGTGCTCAC-3′) was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-γ (IFN-γ) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.

Original language	English
Pages (from-to)	461-469
Number of pages	9
Journal	FEMS Immunology and Medical Microbiology
Volume	46
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-695X.2006.00064.x
Publication status	Published - 2006 Apr

Keywords

Bifidobacterium longum BB536
CpG
Cytokine
Immunoglobulin E
Immunostimulatory sequence oligodeoxynucleotides
Probiotics

ASJC Scopus subject areas

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1574-695X.2006.00064.x

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title = "An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro",

abstract = "The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5′- GCGTCGGTTTCGGTGCTCAC-3′) was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-γ (IFN-γ) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.",

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author = "Noritoshi Takahashi and Haruki Kitazawa and Takeshi Shimosato and Noriyuki Iwabuchi and Xiao, {Jin Zhong} and Keiji Iwatsuki and Sadayuki Kokubo and Tadao Saito",

year = "2006",

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language = "English",

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AU - Takahashi, Noritoshi

AU - Kitazawa, Haruki

AU - Shimosato, Takeshi

AU - Iwabuchi, Noriyuki

AU - Xiao, Jin Zhong

AU - Iwatsuki, Keiji

AU - Kokubo, Sadayuki

AU - Saito, Tadao

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N2 - The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5′- GCGTCGGTTTCGGTGCTCAC-3′) was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-γ (IFN-γ) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.

AB - The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5′- GCGTCGGTTTCGGTGCTCAC-3′) was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-γ (IFN-γ) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.

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KW - Cytokine

KW - Immunoglobulin E

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KW - Probiotics

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An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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