An immunohistochemical study on the ontogeny of cells immunoreactive for spot 35 protein, a novel Purkinje cell-specific protein, in the rat cerebellum

Hiromi Takahashi-Iwanaga, Hisatake Kondo, Tohru Yamakuni, Yasuo Takahashi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

A time course study on the appearance and distribution of cells immunoreactive for spot 35 protein, a novel cerebellar Purkinje cellspecific protein, was conducted in the developing cerebella of fetal and early postnatal rats by PAP immunohistochemistry. Spot 35-immunoreactive cells were first noted in the cerebellar anlage on the 17th embryonic day, appearing as large cellular aggregations in the mantle layer and a small number of elongated cells dispersed between the cell aggregations and the ependymal layer. As the development proceeded, the spot 35-immunoreactive cells gradually accumulated beneath the external granular layer. At birth, they were arranged compactly in 4-5 irregular rows to form a primitive Purkinje cell layer. During their subsequent development, immunostaining for spot 35 protein demonstrated the rearrangement of the Purkinje cells into a single row and the maturation of their somata, axons and dendrites. All these findings indicate that spot 35 protein is a specific marker for the cerebellar Purkinje cells, from their migrating stage throughout the course of their maturation. The present study further describes the transitory lamellar arrangement of spot 35-immunoreactive Purkinje cells located horizontally at a short distance away from the ependymal layer of the cerebellum on the 17th embryonic day.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalDevelopmental Brain Research
Volume29
Issue number2
DOIs
Publication statusPublished - 1986 Oct

Keywords

  • Purkinje cell
  • cerebellum
  • immunohistochemistry
  • ontogeny
  • rat
  • spot 35 protein

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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