An immunohistochemical study of methionine-enkephalin-Arg6-Gly7-Leu8-like immunoreactivity-containing liquor-contacting neurons (LCNs) in the rat spinal cord

Tooru Shimosegawa, Masaru Koizumi, Takayoshi Toyota, Yoshio Goto, Chizuko Yanaihara, Noboru Yanaihara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The localization of methionine-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu)-like immunoreactivity in the medullospinal liquor-contacting neurons (LCNs) of the rat was immunohistochemically investigated using a modified Sternberger's PAP method. In this study, we also examined the distribution of Met-Enk-Arg-Gly-Leu-like immunoreactive LCNs in the entire length of the medulla oblongata and spinal cord. The immunoreactive LCN possessed an oval, polygonal or rod-shaped perikaryon which proturbed an axon-like process and a CSF-contacting process. In the entire length of the medulla oblongata and spinal cord, 60-70 immunoreactive LCNs were observed. They were sparsely distributed along the central canal. The ratio of the number of immunoreactive LCNs in each segment/total number of immunoreactive LCNs in the medulla oblongata and whole spinal cord was as follows: medulla oblongata, 0%; cervical segments, 28.1%; thoracic segments, 56.8%; lumbar segments, 12.0%; sacral segments, 3.0%; and coccygeal segments, 0%. When the immunoreactivity of perikarya of LCNs was weak, a few nerve terminals with a strong Met-Enk-Arg-Gly-Leu-like immunoreactivity were noticed on them. These findings suggest that preproenkephalin A-related opioid peptides including Met-Fnk-Arg-Gly-Leu may be secreted into the cerebrospinal fluid (CSF) through the terminal portions of axon-like processes and/or CSF-contacting processes of LCNs. Enkephalin-containing nerve terminals on the LCNs may represent modulating afferents aiding the receptive and/or secretory functions of the neurons.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBrain research
Volume379
Issue number1
DOIs
Publication statusPublished - 1986 Jul 30

Keywords

  • Immunoheistochemistry
  • medullospinal liquor contacting neuron
  • methionine-enkephalin-Arg-Gly-Leu
  • rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'An immunohistochemical study of methionine-enkephalin-Arg<sup>6</sup>-Gly<sup>7</sup>-Leu<sup>8</sup>-like immunoreactivity-containing liquor-contacting neurons (LCNs) in the rat spinal cord'. Together they form a unique fingerprint.

Cite this