An identification method of a transcription factor binding site using PCR products immobilized on arrays

Motoki Kyo, Terue Kamiya, Tae Suzuki-Yamamoto, Kazuki Inamori, Hozumi Motohashi, Masayuki Yamamoto

Research output: Contribution to conferencePaperpeer-review

Abstract

DNA arrays, on which PCR products with various lengths were immobilized, were fabricated for identification of transcription factor binding site. Surface plasmon resonance (SPR) imaging technique ware applied to detect the bindings between an antioxidant sequence nqo1ARE and transcription factor Nrf2/MafG heterodimer.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
Country/TerritoryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Heterobifunctional hydrophilic crosslinker
  • PCR products
  • Signal normalization
  • Surface plasmon resonance (SPR) imaging technique
  • Transcription factor binding site
  • dsDNA arrays

ASJC Scopus subject areas

  • Engineering(all)

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