An exon 8-spliced out transcript of neurofibromatosis 2 gene is constitutively expressed in various human tissues

Tsutomu Hitotsumatsu, Tetsuyuki Kitamoto, Toru Iwaki, Masashi Fukui, Jun Tateishi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We succeeded in cloning the exon 8-spliced out transcript of neurofibromatosis 2 (NF2) gene in human glioma cell lines. We then investigated the expression of the spliced out form in various human tissues by PCR analysis followed by Southern blot hybridization and sequencing to evaluate whether it was generated by normal alternative splicing or by a splicing mutation. The analysis revealed the splicing out of exon 8 in the various tissues, and the transcript missing exon 8 should thus be considered as a product of normal alternative splicing. Our results further support the possibility that the NF2 gene expresses multiple alternatively-spliced transcripts variantly in different tissue types.

Original languageEnglish
Pages (from-to)1205-1207
Number of pages3
JournalJournal of biochemistry
Volume116
Issue number6
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Keywords

  • Alternative splicing
  • Exon 8
  • Human normal tissues
  • Neurofibromatosis 2

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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