An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation

Max E. Harner, Ann Katrin Unger, Willie J.C. Geerts, Muriel Mari, Toshiaki Izawa, Maria Stenger, Stefan Geimer, Fulvio Reggiori, Benedikt Westermann, Walter Neupert

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38 Citations (Scopus)

Abstract

Metabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present experimental results obtained with yeast that support a novel hypothesis on the existence of two molecular pathways that lead to the generation of lamellar and tubular cristae. Formation of lamellar cristae depends on the mitochondrial fusion machinery through a pathway that is required also for homeostasis of mitochondria and mitochondrial DNA. Tubular cristae are formed via invaginations of the inner boundary membrane by a pathway independent of the fusion machinery. Dimerization of the F1FO-ATP synthase and the presence of the MICOS complex are necessary for both pathways. The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution.

Original languageEnglish
Article numbere18853
JournaleLife
Volume5
Issue numberNOVEMBER2016
DOIs
Publication statusPublished - 2016 Nov 16

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Harner, M. E., Unger, A. K., Geerts, W. J. C., Mari, M., Izawa, T., Stenger, M., Geimer, S., Reggiori, F., Westermann, B., & Neupert, W. (2016). An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation. eLife, 5(NOVEMBER2016), [e18853]. https://doi.org/10.7554/eLife.18853