An epidermal signal regulates Lmx-1 expression and dorsal-ventral pattern during Xenopus limb regeneration

Hiroki Matsuda, Hitoshi Yokoyama, Tetsuya Endo, Koji Tamura, Hiroyuki Ide

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The results of recent studies have supported the idea that the ability to organize the formation of axes such as the anteroposterior and proximodistal axes corresponds to limb regeneration ability in Xenopus. In this study, we investigated the mechanism by which the dorsoventral (D-V) axis of regenerating Xenopus limbs is established and the relationships between D-V patterning and regenerative ability. Transplantation experiments were performed to study which epidermis or mesenchyme is responsible for the D-V patterning in regenerating limbs. Naked mesenchyme of a donor limb was rotated and implanted on a host opposite-side limb stump to make a reversed recombination about the D-V axis. The resultant regenerates had a normal-looking D-V pattern, including Lmx-1 expression, muscle pattern, and joints, in stage 52 recombinants and a reversed D-V pattern in stage 55 recombinants. Further experiments in recombination at stage 52 and stage 55 showed that the epidermal signal is responsible for producing the D-V pattern in the regenerating blastema. These results, together with the finding that Lmx-1 expression is absent in the froglet forelimb blastema, suggest that D-V axis formation is a key step in understanding the loss of regenerative ability.

Original languageEnglish
Pages (from-to)351-362
Number of pages12
JournalDevelopmental Biology
Volume229
Issue number2
DOIs
Publication statusPublished - 2001 Jan 15

Keywords

  • Dorsoventral
  • Limb
  • Lmx-1
  • Regeneration
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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