An epidemiologic survey of methicillin-resistant Staphylococcus aureus by combined use of mec-HVR genotyping and toxin genotyping in a University Hospital in Japan

Junichiro Nishi, Masao Yoshinaga, Hiroaki Miyanohara, Motoshi Kawahara, Masaharu Kawabata, Toshiro Motoya, Tetsuhiro Owaki, Shigeru Oiso, Masayuki Kawakami, Shigeko Kamewari, Yumiko Koyama, Naoko Wakimoto, Koichi Tokuda, Kunihiro Manago, Ikuro Maruyama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

OBJECTIVE: To evaluate the usefulness of an assay using two polymerase chain reaction-based genotyping methods in the practical surveillance of methicillin-resistant Staphylococcus aureus (MRSA). METHODS: Nosocomial infection and colonization were surveyed monthly in a university hospital in Japan for 20 months. Genotyping with mec-HVR is based on the size of the mec-associated hypervariable region amplified by polymerase chain reaction. Toxin genotyping uses a multiplex polymerase chain reaction method to amplify eight staphylococcal toxin genes. RESULTS: Eight hundred nine MRSA isolates were classified into 49 genotypes. We observed differing prevalences of genotypes for different hospital wards, and could rapidly demonstrate the similarity of genotype for outbreak isolates. The incidence of genotype D: SEC/TSST1 was significantly higher in isolates causing nosocomial infections (49.5%; 48 of 97) than in nasal isolates (31.4%; 54 of 172) (P = .004), suggesting that this genotype may represent the nosocomial strains. CONCLUSION: The combined use of these two genotyping methods resulted in improved discriminatory ability and should be further investigated.

Original languageEnglish
Pages (from-to)506-510
Number of pages5
JournalInfection control and hospital epidemiology
Volume23
Issue number9
DOIs
Publication statusPublished - 2002 Sep
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

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    Nishi, J., Yoshinaga, M., Miyanohara, H., Kawahara, M., Kawabata, M., Motoya, T., Owaki, T., Oiso, S., Kawakami, M., Kamewari, S., Koyama, Y., Wakimoto, N., Tokuda, K., Manago, K., & Maruyama, I. (2002). An epidemiologic survey of methicillin-resistant Staphylococcus aureus by combined use of mec-HVR genotyping and toxin genotyping in a University Hospital in Japan. Infection control and hospital epidemiology, 23(9), 506-510. https://doi.org/10.1086/502097