An enzyme probe for the resolution of glucuronoxylan and glucuronoarabinoxylan structures

Kazuhiko Nishitani, Donald J. Nevins

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Recently the analysis of complex carbohydrates has been facilitated by the adoption of an array of sophisticated instrumental techniques. However, the interpretation of native structures based on these methods may be compromised because (i) it is difficult to maintain structural integrity of polysaccharides during their extraction from the cell wall matrix in preparation for analysis and (ii) there are limited definitive approaches for the subsequent allocation of the mixture of extracted components into appropriate homogeneous fractions. Hence a reconciliation of biological function with specific structural entities may be obscured. As an alternative to alkali extraction we describe the use of an enzyme to dissociate fragments of glucuronoarabinoxylan from the cell wall matrix.

    Original languageEnglish
    Pages (from-to)197-207
    Number of pages11
    JournalTopics in Catalysis
    Volume5
    Issue number1-2
    DOIs
    Publication statusPublished - 1991 Jan 1

    ASJC Scopus subject areas

    • Catalysis
    • Chemistry(all)

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