An electrochemical sensor array and its application to real-time brain slice imaging

Nahoko Kasai, Akiyoshi Shimada, Tobias Nyberg, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

An electrochemical sensing system using a planar microelectrode array has been developed for monitoring biological molecules with relatively high spatial and temporal resolution. This allows noninvasive real-time imaging of biological molecules released from tissue. In this study, we establish a multichannel hydrogen peroxide (H2O2) sensing system to monitor the real-time H2O2 distribution in tissue using a planar sensor array. H2O2 is known to be associated with the pathology of neurological diseases because it is a by-product of degenerative reactions involving reactive oxygen species, which are a major cause of oxidative stress in mammalian cells. The sensor array is based on a 64-channel ITO electrode array composed of 50 × 50 μm electrodes modified with an enzyme (horseradish peroxidase) and an electron transfer mediator. We place a cultured rat hippocampal slice on the array and measure the current at each sensor using a multipotentiostat. When we introduce bicuculline into the solution as a stimulant in the presence of a catalase inhibitor, we can observe a distinct increase in the H2O2 concentration. This real-time H2O2 distribution monitoring system is a powerful tool with which to explore the neuronal cell death mechanism in biological systems.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalElectronics and Communications in Japan
Volume92
Issue number9
DOIs
Publication statusPublished - 2009 Sep
Externally publishedYes

Keywords

  • Electrochemical sensor
  • Hippocampal slice
  • Hydrogen peroxide
  • Microelectrode array

ASJC Scopus subject areas

  • Signal Processing
  • Physics and Astronomy(all)
  • Computer Networks and Communications
  • Electrical and Electronic Engineering
  • Applied Mathematics

Fingerprint Dive into the research topics of 'An electrochemical sensor array and its application to real-time brain slice imaging'. Together they form a unique fingerprint.

Cite this