TY - JOUR
T1 - An echo-contrast agent, Levovist, lowers the ultrasound intensity required to induce apoptosis of human leukemia cells
AU - Ando, Hidetaka
AU - Feril, Loreto B.
AU - Kondo, Takashi
AU - Tabuchi, Yoshiaki
AU - Ogawa, Ryohei
AU - Zhao, Qing Li
AU - Cui, Zheng Guo
AU - Umemura, Shin ichiro
AU - Yoshikawa, Hideki
AU - Misaki, Takuro
N1 - Funding Information:
This study is supported in part by the Grant in Aid for Scientific Research on Priority Areas © (12217049) from the Ministry of Education, Culture, Sports, Sciences and Technology; in part by the 21st Century COE Program of the Toyama Medical and Pharmaceutical University, Japan; and also in part by Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine.
PY - 2006/10/8
Y1 - 2006/10/8
N2 - To verify the effect of echo-contrast agent (ECA) on apoptosis induced by ultrasound, leukemia cell lines (Jurkat, Molt-4 and U937) were sonicated at intensities previously shown to induce optimal apoptosis with or without Levovist, an ECA. The results showed that loss of viability and apoptosis can be induced in all three cell lines, apoptosis highest with Molt-4, based on viability and DNA fragmentation assay. Such finding was supported by corresponding increase of cells with low mitochondrial membrane potential, high superoxide production, increased intracellular calcium concentration, and phosphorylation of histone H2AX after sonication. Optimal ultrasound condition was 0.3 W/cm2, 1 MHz, 10% duty factor pulsed at 100 Hz; but in the presence of Levovist, an apparent shift of cell killing induction was observed at 0.2 W/cm2. While these results further confirmed previous findings on ultrasound-induced apoptosis, they also suggest that use of an enhancing factor, such as addition of ECA, may be useful in cancer therapy when a much lower intensity is desired.
AB - To verify the effect of echo-contrast agent (ECA) on apoptosis induced by ultrasound, leukemia cell lines (Jurkat, Molt-4 and U937) were sonicated at intensities previously shown to induce optimal apoptosis with or without Levovist, an ECA. The results showed that loss of viability and apoptosis can be induced in all three cell lines, apoptosis highest with Molt-4, based on viability and DNA fragmentation assay. Such finding was supported by corresponding increase of cells with low mitochondrial membrane potential, high superoxide production, increased intracellular calcium concentration, and phosphorylation of histone H2AX after sonication. Optimal ultrasound condition was 0.3 W/cm2, 1 MHz, 10% duty factor pulsed at 100 Hz; but in the presence of Levovist, an apparent shift of cell killing induction was observed at 0.2 W/cm2. While these results further confirmed previous findings on ultrasound-induced apoptosis, they also suggest that use of an enhancing factor, such as addition of ECA, may be useful in cancer therapy when a much lower intensity is desired.
KW - Apoptosis
KW - Levovist
KW - Ultrasound
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U2 - 10.1016/j.canlet.2005.10.032
DO - 10.1016/j.canlet.2005.10.032
M3 - Article
C2 - 16377079
AN - SCOPUS:33749234351
VL - 242
SP - 37
EP - 45
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -
