An Arf-like Small G Protein, ARL-8, Promotes the Axonal Transport of Presynaptic Cargoes by Suppressing Vesicle Aggregation

Matthew P. Klassen, Ye E. Wu, Celine I. Maeder, Isei Nakae, Juan G. Cueva, Emily K. Lehrman, Minoru Tada, Keiko Gengyo-Ando, George J. Wang, Miriam Goodman, Shohei Mitani, Kenji Kontani, Toshiaki Katada, Kang Shen

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Presynaptic assembly requires the packaging of requisite proteins into vesicular cargoes in the cell soma, their long-distance microtubule-dependent transport down the axon, and, finally, their reconstitution into functional complexes at prespecified sites. Despite the identification of several molecules that contribute to these events, the regulatory mechanisms defining such discrete states remain elusive. We report the characterization of an Arf-like small G protein, ARL-8, required during this process. arl-8 mutants prematurely accumulate presynaptic cargoes within the proximal axon of several neuronal classes, with a corresponding failure to assemble presynapses distally. This proximal accumulation requires the activity of several molecules known to catalyze presynaptic assembly. Dynamic imaging studies reveal that arl-8 mutant vesicles exhibit an increased tendency to form immotile aggregates during transport. Together, these results suggest that arl-8 promotes a trafficking identity for presynaptic cargoes, facilitating their efficient transport by repressing premature self-association.

Original languageEnglish
Pages (from-to)710-723
Number of pages14
JournalNeuron
Volume66
Issue number5
DOIs
Publication statusPublished - 2010 Jun

Keywords

  • Cellbio
  • Molneuro
  • Signaling

ASJC Scopus subject areas

  • Neuroscience(all)

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