An appropriate in vitro culture condition for the induction of human TCRγδ+ cells by heat-killed bacteria

Shuhji Seki, Toru Abo, Hiroshi Sakihara, Keitaro Sugiura, Akihiro Kanno, Hidemi Rikiishi, Takayuki Masuda, Katsuo Kumagai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

TCRγδ+ cells proliferated when MNC were stimulated with various heat-killed bacteria. We investigated here the culture conditions for their maximum proliferation. MNC were cultured for 6 days with Streptococcus pyogenes, and for 3 days with T cell mitogens, PHA and anti-CD3 mAb, in medium supplemented with various concentrations (0.05-50%) of human sera. TCRγδ+ and TCRγδ-CD2+3- double negative cells induced by Str. pyogenes required high concentrations of sera (>6%) for their proliferation. Moreover, increased sera (up to 50%) greatly augmented their proliferation. On the other hand, TCRαβ+ cell proliferation induced by T cell mitogens was supported by small concentration (even 0.1%) of the sera, and the addition of high concentrations of sera (>6%) somewhat suppressed responses. Similar serum requirement patterns were evident for the induction of cytotoxic cells. These results clearly demonstrated the existence of an appropriate culture condition for the proliferation of TCRγδ+ cells induced in vitro.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalJournal of Immunological Methods
Volume139
Issue number1
DOIs
Publication statusPublished - 1991 May 17

Keywords

  • Bacterial stimulation
  • Serum requirement
  • TCRγδ cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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