An AFLP genetic linkage map of pacific abalone (Haliotis discus hannai)

Li Qi, Xu Yanhong, Yu Ruihai, Kijima Akihiro

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A genetic linkage map of Pacific abalone (Haliotis discus hannai) was constructed using AFLP markers based on a two-way pseudo-testeross strategy in a full-sib family. With 33 primer combinations, a total of 455 markers (225 from the female parent and 230 from the male parent) segregated in a 1:1 ratio, corresponding to DNA polymorphism: heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups, equivalent to the H. discus hannai haploid chromosome number, and spanning a total length of 2031.4 cM, with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups, spanning a total length of 2273.4 cM, with an average spacing of 12.9 cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male, on the basis of two estimates of genome length. Fifty-two markers (11.4%) remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs.

Original languageEnglish
Pages (from-to)259-267
Number of pages9
JournalJournal of Ocean University of China
Volume6
Issue number3
DOIs
Publication statusPublished - 2007 Jul

Keywords

  • AFLP
  • Haliotis discus hannai
  • Linkage map
  • Pacific abalone

ASJC Scopus subject areas

  • Oceanography
  • Ocean Engineering

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