An adhesin-like protein, lam29, from lactobacillus mucosae ME-340 binds to histone H3 and blood group antigens in human colonic mucus

Masamichi Watanabe, Hideki Kinoshita, I. Nung Huang, Kei Eguchi, Takuya Tsurumi, Yasushi Kawai, Haruki Kitazawa, Katsunori Kimura, Naoki Taketomo, Daisuke Kikuchi, Tomohiko Sase, Koh Miura, Hitoshi Ogawa, Chikashi Shibata, Akira Horii, Tadao Saito

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A cell-surface 29-kDa protein (Lam29, cysteine-binding protein of the ABC transporter) from Lactobacillus mucosae ME-340 showed an adhesin-like property fo human ABO blood group antigens expressed on the gastrointestinal mucosa. In addition, here we report that Lam29 also bound to an 18-kDa protein on human colonic mucus. By ligand blot assay and N-terminal amino acid sequence of the protein, it was identified as human histone H3. By ligand blot and microplate binding assays with recombinant histone H3, binding between Lam29 and histone H3 was confirmed. The adhesion of ME-340 cells to histone H3 was significantly inhibited by 26% after the addition of 2.5mg/mL Lam29 as compared to the absence of Lam29 (p < 0:01). By GHCl extraction and transcription attenuation of ME-340 cells, binding reduction of ME340 cells against histone H3 was detected at 12% and 13% respectively, as compared to control cells by the BIACORE assay (p < 0:01). These data indicate that Lam29 shows multiple binding activities to blood group antigens and histone H3 in human colonic mucus. This is the first report to indicate that lactobacilli expressing Lam29 adhere to histone H3 on gastrointestinal mucosa.

Original languageEnglish
Pages (from-to)1655-1660
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number9
DOIs
Publication statusPublished - 2012

Keywords

  • ABC transporter
  • Adhesion
  • Histone H3
  • Human colonic mucus
  • Lactobacillus

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'An adhesin-like protein, lam29, from lactobacillus mucosae ME-340 binds to histone H3 and blood group antigens in human colonic mucus'. Together they form a unique fingerprint.

Cite this