Amplification of c-Ki-ras gene and aberrant expression of c-myc in WI-38 cells transformed in vitro by γ-irradiation

Kiyoshi Mizuki, Kiyoshi Nose, Hiroshi Okamoto, Nobuo Tsuchida, Kenshi Hayashi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The expression of c-oncogenes was examined with normal human fibroblasts (WI-38) and the cells transformed in vitro by γ-irradiation (CT-1). The amount of Ki-ras-specific mRNA in CT-1 cells was found to be approximately twice that in WI-38 cells. By Southern blot hybridization, the c-Ki-ras 2 gene of CT-1 cells was found to be amplified about two-fold. CT-1 cells have higher levels of c-myc mRNA than normal cells, although the gene dosage and the restriction nuclease pattern of the c-myc gene was the same. The content of c-myc mRNA in CT-1 cells was found to be constitutively high, whereas that in normal cells was increased by serum addition.

Original languageEnglish
Pages (from-to)1037-1043
Number of pages7
JournalBiochemical and biophysical research communications
Volume128
Issue number2
DOIs
Publication statusPublished - 1985 Apr 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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