AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle

Masato Miyake, Hideyuki Takahashi, Eri Kitagawa, Hitoshi Watanabe, Takahiro Sakurada, Hisashi Aso, Takahiro Yamaguchi

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    AMP-activated protein kinase (AMPK) regulates metabolism in skeletal muscle and myostatin (MSTN) negatively regulates skeletal muscle development and growth. In the present study, AMPK activation and the relationship between AMPK and MSTN during myogenic differentiation were investigated in cultures derived from bovine skeletal muscle. Myoblasts capable of forming myotubes were obtained from bovine skeletal muscle and treated with AICAR to activate AMPK, resulting in suppressed myotube formation. AICAR treatment significantly reduced the expression of MSTN mRNA during myogenic differentiation. Combined treatment with AICAR and MSTN suppressed myotube formation to a greater extent than AICAR alone. SB431542, an inhibitor of MSTN signaling, promoted myotube formation during myogenic differentiation. However, simultaneous treatment with AICAR blocked this effect of SB431542. Therefore, AMPK activation inhibits myogenic differentiation but may suppress MSTN expression to balance muscle development.

    Original languageEnglish
    Pages (from-to)615-623
    Number of pages9
    JournalCell and Tissue Research
    Volume349
    Issue number2
    DOIs
    Publication statusPublished - 2012 Aug

    Keywords

    • AMPK
    • Cattle
    • Differentiation
    • Myoblast
    • Myostatin

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Histology
    • Cell Biology

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