Amphipaths differentially modulate membrane surface deformation in rat peritoneal mast cells during exocytosis

Itsuro Kazama, Yoshio Maruyama, Sara Takahashi, Takashi Kokumai

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Background/Aims: Salicylate and chlorpromazine exert differential effects on the chemokine release from mast cells. Since these drugs are amphiphilic and preferentially partitioned into the lipid bilayers of the plasma membranes, they would induce some morphological changes in mast cells and thus affect the process of exocytosis. Methods: Employing the standard patch-clamp whole-cell recording technique, we examined the effects of salicylate and chlorpromazine on the membrane capacitance (Cm) during exocytosis in rat peritoneal mast cells. Using confocal imaging of a water-soluble fluorescent dye, lucifer yellow, we also examined their effects on plasma membrane deformation of the cells. Results: Salicylate dramatically accelerated the GTP-γ-S-induced increase in the Cm immediately after its application, whereas chlorpromazine significantly suppressed the increase. Treatment with salicylate increased the trapping of the dye on the cell surface, while treatment with chlorpromazine completely washed it out, indicating that both drugs induced membrane surface deformation in mast cells. Conclusion: This study demonstrated for the first time that membrane amphipaths, such as salicylate and chlorpromazine, may oppositely modulate the process of exocytosis in mast cells, as detected by the changes in the Cm. The plasma membrane deformation induced by the drugs was thought to be responsible for their differential effects.

Original languageEnglish
Pages (from-to)592-600
Number of pages9
JournalCellular Physiology and Biochemistry
Volume31
Issue number4-5
DOIs
Publication statusPublished - 2013 May

Keywords

  • Chlorpromazine
  • Exocytosis in mast cells
  • Membrane capacitance
  • Plasma membrane deformation
  • Salicylate

ASJC Scopus subject areas

  • Physiology

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