Amphioxus connectin exhibits merged structure as invertebrate connectin in I-Band region and vertebrate connectin in A-band region

Satoshi Ohtsuka, Akira Hanashima, Kaoru Kubokawa, Yulong Bao, Yukiko Tando, Junki Kohmaru, Hiroyuki Nakaya, Koscak Maruyama, Sumiko Kimura

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Connectin is an elastic protein found in vertebrate striated muscle and in some invertebrates as connectin-like proteins. In this study, we determined the structure of the amphioxus connectin gene and analyzed its sequence based on its genomic information. Amphioxus is not a vertebrate but, phylogenetically, the lowest chordate. Analysis of gene structure revealed that the amphioxus gene is approximately 430 kb in length and consists of regions with exons of repeatedly aligned immunoglobulin (Ig) domains and regions with exons of fibronectin type 3 and Ig domain repeats. With regard to this sequence, although the region corresponding to the I-band is homologous to that of invertebrate connectin-like proteins and has an Ig-PEVK region similar to that of the Neanthes sp. 4000K protein, the region corresponding to the A-band has a super-repeat structure of Ig and fibronectin type 3 domains and a kinase domain near the C-terminus, which is similar to the structure of vertebrate connectin. These findings revealed that amphioxus connectin has the domain structure of invertebrate connectin-like proteins at its N-terminus and that of vertebrate connectin at its C-terminus. Thus, amphioxus connectin has a novel structure among known connectin-like proteins. This finding suggests that the formation and maintenance of the sarcomeric structure of amphioxus striated muscle are similar to those of vertebrates; however, its elasticity is different from that of vertebrates, being more similar to that of invertebrates.

Original languageEnglish
Pages (from-to)415-426
Number of pages12
JournalJournal of Molecular Biology
Volume409
Issue number3
DOIs
Publication statusPublished - 2011 Jun 10

Keywords

  • Fn
  • JGI
  • Joint Genome Institute
  • PBS
  • fibronectin type 3
  • phosphate-buffered saline

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Amphioxus connectin exhibits merged structure as invertebrate connectin in I-Band region and vertebrate connectin in A-band region'. Together they form a unique fingerprint.

Cite this