Amperometric uric acid sensors based on polyelectrolyte multilayer films

Tomonori Hoshi, Hidekazu Saiki, Jun Ichi Anzai

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Uricase (UOx) and polyelectrolyte were used for preparation of a permselective multilayer film and enzyme multilayer films on a platinum (Pt) electrode, allowing the detection of uric acid amperometrically. The polyelectrolyte multilayer (PEM) film composed of poly(allylamine) (PAA) and poly(vinyl sulfate) (PVS) were prepared via layer-by-layer assembly on the electrode, functioning as H2O2-selective film. After deposition of the permselective film (PAA/PVS)2PAA, UOx and PAA were deposited via layer-by-layer sequential deposition up to 10 UOx layers to prepare amperometric sensors for uric acid. Current response to uric acid was recorded at +0.6 V vs. Ag/AgCl to detect H2O2 produced from the enzyme reaction. The response current increased with increasing the number of UOx layers. Even in the presence of ascorbic acid, uric acid can be detected over the concentration range 10-6-10-3 M. The response current and deposited amount of UOx were affected by deposition bath pH and the addition of salt. The deposition of PAA/UOx film prepared in 2 mg ml-1 solution (pH 11) of PAA with NaCl (8 mg ml-1) and 0. 1 mg ml-1 solution (pH 8.5) of UOx with borate (100 mM) resulted in an electrode which shows the largest response to uric acid. The response of the sensor to uric acid was decreased by 40% from the original activity after 30 days.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalTalanta
Volume61
Issue number3
DOIs
Publication statusPublished - 2003 Nov 4

Keywords

  • Amperometric measurement
  • Polyelectrolyte
  • Uric acid sensors

ASJC Scopus subject areas

  • Analytical Chemistry

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