TY - JOUR
T1 - Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device
AU - Zhu, Xi
AU - Ino, Kosuke
AU - Lin, Zhenyu
AU - Shiku, Hitoshi
AU - Chen, Guonan
AU - Matsue, Tomokazu
N1 - Funding Information:
This study was partly supported by the Formation of Innovation Center for Fusion of Advanced Technologies, Special Coordination Funds for Promoting Science and Technology, and by Grant-in-Aid for Scientific Research ( 22245011 ) from the Ministry of Education, Culture, Sports, Science and Technology , Japan. This study was also supported by the CASIO Science Promotion Foundation . We also thank the National Nature Sciences Funding of China for their support ( 20735002 , 41076059 and 20905013 ).
PY - 2011/12/15
Y1 - 2011/12/15
N2 - A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 × 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)64-/Fe(CN)63- at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 μM) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species.
AB - A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 × 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)64-/Fe(CN)63- at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 μM) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species.
KW - Addressable
KW - DNA detection
KW - Electrochemical detection
KW - Multipoint detection
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U2 - 10.1016/j.snb.2011.09.004
DO - 10.1016/j.snb.2011.09.004
M3 - Article
AN - SCOPUS:81155134462
VL - 160
SP - 923
EP - 928
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1
ER -