Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device

Xi Zhu; Kosuke Ino; Zhenyu Lin; Hitoshi Shiku; Guonan Chen; Tomokazu Matsue

doi:10.1016/j.snb.2011.09.004

Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device

Xi Zhu, Kosuke Ino, Zhenyu Lin, Hitoshi Shiku, Guonan Chen, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 × 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)₆^4-/Fe(CN)₆^3- at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 μM) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species.

Original language	English
Pages (from-to)	923-928
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2011.09.004
Publication status	Published - 2011 Dec 15

Keywords

Addressable
DNA detection
Electrochemical detection
Multipoint detection

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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abstract = "A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 × 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)64-/Fe(CN)63- at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 μM) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species.",

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AU - Lin, Zhenyu

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AU - Chen, Guonan

AU - Matsue, Tomokazu

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