Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device

Xi Zhu, Kosuke Ino, Zhenyu Lin, Hitoshi Shiku, Guonan Chen, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 × 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)64-/Fe(CN)63- at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 μM) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species.

Original languageEnglish
Pages (from-to)923-928
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume160
Issue number1
DOIs
Publication statusPublished - 2011 Dec 15

Keywords

  • Addressable
  • DNA detection
  • Electrochemical detection
  • Multipoint detection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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