Amperometric biosensor based on tyrosinase-conjugated polysacchride hybrid film: Selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid

Aihua Liu, Itaru Honma, Haoshen Zhou

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

The amperometric detection of neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) was achieved at a tyrosinase-chitosan composite film-modified glassy carbon (GC) electrode. The optimal conditions for the preparation of the biosensor were established. This bio-composite film was characterized by scanning electron microscopy (SEM) and Fourier transformed infrared (FT-IR) spectra, suggesting that chitosan covalently connected to chitosan chains. Electrochemical characterization of the bio-hybrid membrane-covered electrodes were also performed in 0.05 M phosphate buffer solution (pH 6.52) containing neurotransmitters or their derivatives by using cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and amperometry. This simply-prepared protein-polysaccharide hybrid film provides a microenvironment friendly for enzyme loading. The sensor was operated at -0.15 V with a short response time. The current linearly increased with the increasing concentration of DOPAC over the concentration of 6 nM-0.2 mM. The lower detection limit for DOPAC is 3 nM (S/N = 3). The sensitivity of the sensor is 40 μA mM-1. A physiological level of neurotransmitters and their derivatives including dopamine, l-dopa, adrenaline, noradrenaline and homovanillic acid as well as ascorbic acid, uric acid and acetaminophen do not affect the determination of DOPAC.

Original languageEnglish
Pages (from-to)809-816
Number of pages8
JournalBiosensors and Bioelectronics
Volume21
Issue number5
DOIs
Publication statusPublished - 2005 Nov 15
Externally publishedYes

Keywords

  • Amperometric biosensor
  • Chitosan
  • DOPAC
  • Neurotransmitters
  • Selective detection
  • Tyrosinase

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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