Amperometric Alcohol Sensors Based on Protein Multilayers Composed of Avidin and Biotin-Labeled Alcohol Oxidase

Xiao Yan Du, Jun Ichi Anzai, Tetsuo Osa, Ryoichi Motohashi

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Protein multilayers composed of avidin and biotin-labeled alcohol oxidase (AOx) were prepared on the surface of a platinum (Pt) electrode by repeated deposition of the proteins to develop amperometric alcohol sensors. The AOx multilayer-modified sensors showed a rapid response to ethanol over the concentration range of 0.01-10mg/10mL. The output current of the sensor depended linearly upon the thickness of the AOx layers (or the number of the AOx layers), suggesting that a constant amount of AOx can be immobilized by each deposition. The selectivity of the sensor to aliphatic alcohols was in the order of methanol > ethanol > n-propanol > n-butanol, being in accord with the selectivity of AOx in a homogeneous system itself. The sensor retained its original response to ethanol for about one month without deterioration.

Original languageEnglish
Pages (from-to)813-816
Number of pages4
JournalElectroanalysis
Volume8
Issue number8-9
DOIs
Publication statusPublished - 1996

Keywords

  • Alcohol
  • Avidin
  • Biosensor
  • Biotin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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