Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol

Hiraku Sasaki, Jun Nonaka, Takako Sasaki, Yutaka Nakai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4′,6′-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalJournal of Industrial Microbiology and Biotechnology
Volume34
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

Keywords

  • Ammonia
  • Compost
  • Lagoon
  • Manure
  • PVA immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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