AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene

Hitoshi Okada, Toshio Watanabe, Masaru Niki, Hiroshi Takano, Natsuko Chiba, Nobuaki Yanai, Kenzaburo Tani, Hitoshi Hibino, Shigetaka Asano, Michael L. Mucenski, Yoshiaki Ito, Tetsuo Noda, Masanobu Satake

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

The AML1 and PEBP2β/CBFβ genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of definitive hematopoiesis in the fetal liver on embryonic day 11.5 (E11.5). We examined and compared the expression levels of various hematopoiesis-related genes in wild type embryos and in embryos mutated for AML1 or PEBP2β/CBFβ. The RNAs were prepared from the yolk sacs of E9.5 embryos, from the aorta-gonad- mesonephros regions of E11.5 embryos and from the livers of E11.5 embryos and RT-PCR was performed to detect various gene transcripts. Transcripts were detected for most of the hematopoiesis-related genes that encode transcription factors, cytokines and cytokine receptors, even in tissues from homozygously targeted embryos. On the other hand, PU.1 transcripts were never detected in any tissue of AML1(-/-) or PEBP2β/CBFβ(-/-) embryos. In addition, transcripts for the Vav, flk-2/flt-3, M-CSF receptor G-CSF receptor and c-Myb genes were not detected in certain tissues of the (-/-) embryos. The results suggest that the expression of a particular set of hematopoiesis-related genes is closely correlated with the PEBP2/CBF function.

Original languageEnglish
Pages (from-to)2287-2293
Number of pages7
JournalOncogene
Volume17
Issue number18
DOIs
Publication statusPublished - 1998 Nov 5

Keywords

  • AML1
  • Gene targeting
  • Hematopoiesis
  • PEBP2β/CBFβ
  • PU.1
  • RT-PCR
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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