Amelioration of vasospasm after subarachnoid hemorrhage in transgenic mice overexpressing CuZn-superoxide dismutase

Hideyuki Kamii, Ichiro Kato, Hiroyuki Kinouchi, Pak H. Chan, Charles J. Epstein, Atsushi Akabane, Hiroshi Okamoto, Takashi Yoshimoto

Research output: Contribution to journalArticlepeer-review

111 Citations (Scopus)

Abstract

Background and Purpose-To clarify the effect of superoxide dismutase (SOD) on vasospasm after subarachnoid hemorrhage (SAH), we investigated sequential changes in arterial diameter after SAH in transgenic mice overexpressing CuZn-SOD (SOD-1). Methods-SOD-transgenic mice and nontransgenic littermates (35 to 40 g) were subjected to SAH produced by endovascular perforation of left anterior cerebral artery. At 4 hours and 1, 3, 7, and 14 days after SAH, the mice were perfused with 10% formalin and consequently with a mixture of carbon black and 10% gelatin to cast all vessels. Vasospasm was evaluated by measuring the diameter of the left middle cerebral artery (MCA) with a microscope. Results-In nontransgenic mice, the diameter of the MCA on day 3 after SAH (110.5±20.5 μm [mean±SD]; n = 16) was significantly reduced compared with that without SAH (138.5 ± 14.5 μm; n=12) (P<0.01). Moreover, on day 3 after SAH, the diameter of the MCA in SOD- transgenic mice (127.9±20.2 μm; n=20) was significantly larger than that in nontransgenic mice (110.5±20.5 μm; n= 16) (P<0.05). Conclusions-These results suggest that SOD is effective on the amelioration of vasospasm after SAH and that oxygen free radicals, particularly superoxide, play an important role in the pathogenesis of vasospasm after SAH.

Original languageEnglish
Pages (from-to)867-872
Number of pages6
JournalStroke
Volume30
Issue number4
DOIs
Publication statusPublished - 1999 Apr

Keywords

  • Free radicals
  • Mice, transgenic
  • Subarachnoid hemorrhage
  • Superoxide dismutase
  • Vasospasm

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialised Nursing

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