Amadori-glycated phosphatidylethanolamine induces angiogenic differentiations in cultured human umbilical vein endothelial cells

Jeong Ho Oak, Kiyotaka Nakagawa, Shinichi Oikawa, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Glycation has been implicated in the endothelial dysfunction that contributes to both diabetes- and aging-associated vascular complications. The aim of the present study was to determine whether Amadori-glycated phosphatidylethanolamine (Amadori-PE), a lipid-linked glycation compound that is formed at an increased rate in hyperglycemic states, affected proliferation, migration and tube formation of cultured human umbilical vein endothelial cells (HUVEC). Amadori-PE at a low concentration of less than 5 μM significantly enhanced these three factors involved in angiogenesis. Furthermore, stimulation of HUVEC with Amadori-PE resulted in secretion of matrix metalloproteinase 2 (MMP-2), a pivotal enzyme in the initial step of angiogenesis. Our results demonstrated for the first time that Amadori-PE may be an important compound that promotes vascular disease as a result of its angiogenic activity on endothelial cells. We also demonstrated that MMP-2 is a primary mediator of Amadori-PE-driven angiogenesis.

Original languageEnglish
Pages (from-to)419-423
Number of pages5
JournalFEBS Letters
Volume555
Issue number2
DOIs
Publication statusPublished - 2003 Dec 4

Keywords

  • Amadori product
  • Angiogenesis
  • Diabetes mellitus
  • Glycation
  • Matrix metalloproteinase
  • Phosphatidylethanolamine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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